Between 14/11/2022 to 19/11/2022 (USJ – 1st part), a repetition of TP2 (1st part) related to “Industrial microbiology, fermentation and scale-up”was realised with 12 MSc students at USJ (M2, Technologie Industrielle, USJ) divided into 5 groups.
Scientific and technical objectives were :
- Basic knowledge related with bioprocess engineering (biokinetics, stoechiometry, cell culture in bioreactor, operating modes, transfer limitation,…)
- Cell cultures in bioreactor (TP1) including: (i) experimental environment (bioreactor, analytical methods,…), (ii) data acquisition and treatment.
- Data treatment and interpretation (global and instantaneous balances, mass, elementary and redox balances, kinetics analysis, gas transfer,…).
This experimental activity with Erlenmeyers (E. coli, Bt kurstaki LIP) and 1 bioreactor (Bt kurstaki LIP) were conducted in batch mode. Experiments have been conducted with minimal mineral (E.coli) or semi-synthetic medium (Bt LIP). The experimental objectives were:
- To inventory all information collected during cell culture in bioreactor
- To illustrate key points (Bioreactors: preparation and cell culture, biochemical analytical capacity and Analytical capacity, data monitoring and overview of experimental notes.)
To give a global overview of cell culture: (i) evolution of physico-chemical parameters and (ii) evolution of biotics data.
