IPM-4-CITRUS @ International Days of Biotechnology in Sousse, Tunisia – 18th to 22nd Dec. 2019

Next December will be another opportunity for the IPM-4-CITRUS project and consortium to communicate about the results and leads of the project while participating in the International Days of Biotechnology, organised by the Tunisain Biotechnology Association (A.T. Biotech) in Sousse… https://www.atbiotech.org/eng/home

This event will fous on the following main scientific themes:

  • Industrial Biotechnology : Enzymes, microorganisms, metagenomics and bioprocesses.
  • Environmantal Biotechnology : Waste treatment and recovery, bioremediation, bioenergy and phytoremediation.
  • Plant Biotechnology : Genetic diversity, environmental constraints and plant improvement, and plant-microorganism interaction.
  • Health Biotechnologie  : Vaccines and diagnostics.
  • Bioactive Molécules and applications.

A dedicated 2h30-slot on 19th December (morning) will be for IPM-4-CITRUS the opportunity to let its young researchers and seconded staff shine through their presentations!

Scientific exchange – High school Ste Marie des Champs (May 2019) – in relation with durable agriculture

On Friday 17th May 2019, a debate around durable agriculture was organised with 28 students (Première scientifique) from High school Ste Marie des Champs (May 2019). Exchange was organised around two moments:
• Firstly, an introductive game around a central question “What do you think about Biopesticides ?” was realised.
All participants were engaged to play a role (scientist, consumer, farmer, industrial, and policy-maker – identified by coloured post-it) and to express their position (Poor / negative, Average, Positive / Excellent) considering 4 topics (Health & Environment, Price & Profitability, Efficiency, Political & Social Readiness).
All these information are synthesised on a specific board and used during exchange with audience.
• Secondly, students have worked on subjects related to durable agriculture (ex. intensive agriculture, human feeding and ecosystem, agro forestry,…). Flash presentations (5-10 minutes) and exchange with jury were conducted.

Several events (RT1, RT3, ESOF, Highschool Ste Marie) have been driven around the questions “What do you think about Biopesticides ?”.

A synthesis of exchanges and arguments per role (farmer, consumer, policy maker, industry, scientist) has consequently be done. Exchanges were really fruitful to evaluate argument pro and contra biopesticide acceptation as a function of audience and played roles.

3rd IPM-4-CITRUS Round Table @ University of Saint-Joseph, Beirut Lebanon

On 7th March 2019, nearly 120 people from Civil Society gathered at host institution and project partner University of Saint-Joseph, in Beirut, Lebanon, to attend IPM-4-CITRUS H2020 funded project 3rd Round Table about the acceptability of Biopesticides.
The Round Table kicked in with the now traditional “Ice-breaker” game around Biopesticide and a serie of presentations by 3 Lebanese experts:

  1. Mrs. Pauline EID – Agriculture Engineer at the Plant Protection Department – Coordinator of the organic agriculture technical committee, Lebanese Ministry of Agriculture – Lebanon “Organic Agriculture in Lebanon”
  2. Eng. Mohammad Abu Zeid – Director of plant resources, Ministry of Agriculture “Biopesticide registration in Lebanon”
  3. Mr. Jad DANDASH Agricultural Economist & Consultant, Freelance “Eco-Librium”

Those speeches gave a better insight of the Lebanese view and positioning around Biopesticides, Organic Agriculture but also Market regulations and enabled to nurture the main round table exchanges and project presentation. The high-quality of the debates and questions raised, as well as the huge number of attendees again demonstrate that the IPM-4-CITRUS project is targeting a hot topic, contributing to increase stakeholder’s awareness.

ESOF Toulouse, conference Session Biopesticides , July 2018

ESOF (EuroScience Open Forum), Toulouse, July 2018


Open innovation for biopesticides: a new paradigm**  

Place : Pierre Baudis International Congress Centre, Toulouse – France

Although traditional innovation used to be a vertical process within companies, a new open innovation paradigm has emerged with a triple-helix model involving interactions between policy-makers, academia and industry and even a quadruple helix with the growing involvement of civil society. In this context, the Horizon2020-funded IPM-4-CITRUS project uses an interdisciplinary, intersectoral and international approach to optimise bioproduction processes and develop new biopesticides in the Mediterranean region. By proposing a feasibility study for future spin-off activities and new production lines, the project is directly confronted with the opportunities & obstacles related to bringing innovative ideas to the market. Through the innovative concept of « Interactive Panel » we propose to stimulate ideas from the audience and create a real-life open innovation platform to build up a SWOT analysis of biopesticide innovation. Upon entering the room, the public will be provided with coloured post-its with sentences regarding intersectoral interactions in the field of biopesticides. For a few minutes, they will classify the post-its on paper-boards according to the SWOT matrix and a special poster will be devoted to questions. 5-6 panelists from various countries (France, Tunisia, Lebanon, Turkey) and sectors (academia, industry) will then be asked to pick random topics, present their own perspectives and react to the audience’s first classification. Examples of questions to be tackled are: how can interdisciplinarity benefit/hinder the development of biopesticides? What role can policy-making play? How can civil society be heard? Which economic model could integrate all stakeholders’ expectations? After this discussion, time will be devoted to answering questions. Through interactive exchanges, the participants will learn about the challenges of bringing innovative biopesticides to the market and starting new businesses in this field in various countries.

Submitted by Luc FILLAUDEAU – LISBP, INSAT and Jerome CUBERO – INSA Toulouse / L.I.S.B.P.

Speakers :
Luc FILLAUDEAU, Project leader – INRA Toulouse, LISBP / INSA Toulouse
Souad ROUIS, Project co-leader – Centre Biotechnologie de Sfax, Tunisie (CBS)
Nadia BEN SAID – Project Partner – Laboratoires MEDIS, Tunisie
Jerome CUBERO, IPM-4-CITRUS Project Manager – INSA Toulouse
Laure FABRE, European Project Coordinator – INSA Toulouse


Training TP2


Training TP2 is dedicated to “Industrial microbiology, fermentation and scale-up” associated with a Demo Day on “Scale up (from Erlenmeyer to bioreactor) and product recovery”, the consortium has acted to realised it at CBS (at valorisation unit, Sfax, Tunisia) instead of LISBP (Toulouse, France). By this way, it will be easier to train engineers from CBS and MEDIS in association with 2 secondments (FILLAUDEAU Luc and ACEVES-LARA César).

TP2 was extended to 2 weeks:

• Week WK26: cell culture in bioreactor (with FILLAUDEAU Luc)

• Week WK43: data treatments and bio-kinetics modelling (with ACEVES-LARA César)

Scientific and technical objectives are:

  • Basic knowledge related with bioprocess engineering (biokinetics, 6toichiometry, cell culture in bioreactor, operating modes, transfer limitation,…)
  • Cell cultures in bioreactor (TP1) including: (i) experimental environment (bioreactor, analytical methods,…), (ii) data acquisition and treatment.
  • Data treatment and interpretation (global and instantaneous balances, mass, elementary and redox balances, kinetics analysis, gas transfer,…).

8 persons have been registered (effective participants limited to 6) in this training T2:

• From MEDIS : Tayssir HAMMAR (IR)

• From valorisation unit (CBS) : Adel ZITOUN, Faicel BOUKHILI, Najoua AYADI

• From Biopesticide team (CBS): Fati HELTELLY, Wafa JALLOULI, 

• From ENIS : Zeinep BAKLOUTI (student ENIS4)

During the 1st week, the experimental activity with 2 bioreactors (3 and 7L) was conducted with a model microorganism (E. coli in batch mode) and with Bt (Tunisian strain BLB1 in batch / fed-bath mode). Experiments have been conducted with minimal mineral (E.coli) or semi-synthetic medium (Bt BLB1). Several aspects have been validated such as the synthetic medium with Bt BLB1 as well its concentrated formulation.


o To inventory all information collected during cell culture in bioreactor

o To illustrate key points (Bioreactors: preparation and cell culture, biochemical analytical capacity and Analytical capacity, data monitoring and overview of experimental notes.)

o To give a global overview of cell culture: (i) evolution of physico-chemical parameters and (ii) evolution of biotics data.


Ce diaporama nécessite JavaScript.


Training TP1

(20th April 2018, CBS, Sfax): “Biocatalyst improvement & bioreactor cultivation: from basic concept up to intensified bioproduction”

PM1 was associated with 2 satellites events (TP1 and RT1), Training TP1 is briefly described hereafter.

Secondments have been planned in relation to each partner’s skills and expertise and the most relevant meeting institution as regards knowledge sharing and project activities. In WP2, exchanges between CBS and FS-USJ will be essential to share knowledge about the two considered strains, in relation to INSAT and TWB’s expertise for bioreactor and pre-industrial scale-up. This is coupled with two distinct training periods (TP1 and TP2), which will enable INSAT to share its knowledge and expertise to the whole consortium as well as scientists within the partner institutions in a more formalised way.

TP1 was a conference & theory training focussed on “Biocatalyst improvement & bioreactor cultivation: from basic concept up to intensified bioproduction” which has involved 3 experts from LISBP and TWB (ACEVES-LARA César, PICOT Marc and FILLAUDEAU Luc). 

• Items : “Biocatalyst improvement & bioreactor cultivation: from basic concept up to intensified bioproduction”

• Objectives :

o Culture for Bioreactors (management, instrumentation, modelling)

o Operations, set ups & Technological range

o Sensors & Actuators for Bioprocesses

o Modelling of Bioprocesses

• Audience / Participants: scientist involved in IPM-4-Citrus (25 persons)

• Associated documents: list of participants and associated documents (pptx, video)

This TP1 was also discussed by project supervision committee with WP leaders. Considering the content of TP1, the objectives were to optimise the impact of TP2 (place, date and duration and audience) in agreement with IPM-4-Citrus. Considering Training TP2 “Industrial microbiology, fermentation and scale-up” associated with a Demo Day on “Scale up (from Erlenmeyer to bioreactor) and product recovery”, the consortium has acted to realised it at CBS (at valorization unit, Sfax, Tunisie) instead of LISBP (Toulouse, France). By this way, it will be easier to train engineers from CBS and MEDIS in association with 2 secondments (FILLAUDEAU Luc and ACEVES-LARA César). TP2 will also be extended to 2 weeks:

• Week 26: cell culture in bioreactor (with FILLAUDEAU Luc)

• Week 43: biokinetics modelisation (with ACEVES-LARA César)

A similar strategy could be conducted at USJ and will be discussed during Mid Term meeting.


Roundtable RT1

PM1 was associated with 2 satellites events (TP1 and RT1), Round Table RT1 is briefly described hereafter. RT1 was initially scheduled at FS-USJ in M6. Due to delay in the project, this RT was realised at CBS in association with PM1 (M12).

A world café is an innovative method of conversation that stimulates participants’ creativity and allows them to exchange ideas. First, through a lively, video presentation, participants will be introduced to Bacillus thuringiensis (Bt), a microorganism used in citrus pest management around the Mediterranean region. The different speakers will come from a variety of countries (France, Tunisia, Turkey, Italy) and sectors (academia, industry, NGOs). Then, through role-playing in different round tables chaired by the different speakers, the participants will impersonate representatives of each of the sectors involved (scientists, industrial producers/suppliers, farmers, consumers, policy-makers, etc.). They will then argue with other participants (representing other stakeholders) in order to provide a SWOT analysis of the introduction of Bt into agricultural practices. In a final phase, each round table will present its conclusions to the audience in poster form. Through this role-playing and these interactive exchanges, all the participants will be sensitised to alternatives to chemical pesticides and the challenges linked to the biopesticides.

Our RT1 has considered the central questioning for IPM : “What are the scientific and socio-economic impacts of the project ?”. RT1 has generated a participative science/collective debate and was organised a “World café” event. The rules were: (i) 4 groups (8 to 12 persons), (ii) 1 role par person or group (randomly chosen) as scientists, industrial producers/suppliers, farmers, consumers, policy-makers, (iii) 1 animator/moderator and (iv) each group conducts a SWOT analyse related to IPM. At the end, collective data and results recovery & conclusion was realised. CUBERO Jerome (EU project manager, INSAT) acted as moderator, and FILLAUDEAU Luc (DR INRA, LISBP), ROUIS Souad (PR CBS, Sfax), TOUNSI Slim (PR CBS, Sfax) and KHALLASSY Mireille (USJ, Libenon) as experts and group animators.

Items : Acceptability of Biopesticides by civil society

The main step of Round Table were :

  • Short introduction about IPM-4-Citrus, Citrus Disease Integrated Pest Management: from Research to Market
  • Video : Presentation of Biopesticides, Production & Application
  • Participative “World café”: Acceptability of Biopesticides by civil society (opened discussion)
  • Collective conclusion

Audiences / Participants : academic, industrial and civil society (env. 40 persons)

A global synthesis of the main exchanges and arguments per role has consequently be done (Table). Exchanges were really intense as RT1 lasts 2.5h instead of 1h. The consortium plans to take part in the ESOF 2018 (Toulouse, July 2018) and will therefore try to perfectly match its motto: “Sharing Science: towards new horizons”. If selected, the partners will apply for the ESOF label, granted based on the following criteria: (i) scientific excellence; (ii) link with society and societal impact; (iii) European dimension; (iv) young people’s involvement. Our submitted proposal was selected, and the results from RT1 were used as support.

Ce diaporama nécessite JavaScript.

Progress meeting #1 Sfax, April 2018

IPM-4-Citrus PM1 meeting took place on 19th and 20th April 2018 at CBS (Sfax, Tunisia) in pleasant and open exchange atmosphere. All consortium partners (23 persons) were present in addition to staff members associated to communication & dissemination, financial and administrative management. Exchanges and questions have been related as well as reminder to (i) RISE operating mode, (ii) the discovery of EU Portal, IPM-4-Citrus workspace and Website, (iii) modification related to GA amendment and (iv) scientific & technical progress.

Scientific and technical aspects (WP2 and WP4) associated with secondments (Internal reports, IR) :

  • Bibliographic review related to Bt kurstaki (focus on BLB1 et LIP)
  • Sharing information of Materials & Methods (between CBS, USJ, LISBP, TWB)
  • Comparison of strains and industrial mediums (lab scale)
  • Initiate the technical-economical analyses

Scientific presentations:

  • Wafa JALLOULI IR2.1 (Bibliography), è Associated to Endnote base and literature review, PU)
  • Nouha  ABDELMALEK IR2.1 (Mat&Meth)
  • Fatma DRISS, Ilem HSARI IR2.2 (WB, BLB1 and LIP)
  • Nouha ABDELMALEK R2.2 (WB, BLB1 and LIP)

IR2.1 and 2.2 will be consolidated into D2.1 (PU) after PM1 discussion

  • Sara FALVO & Bernadetta MARESCA IR4.1 consolidated into D4.1

Ce diaporama nécessite JavaScript.

Kick of Meeting – 27-28 April 2017

During Kick – Off Meeting (KOM) the following key points will be presented and discussed:

  • To gather consortium members and discuss details on project implementation
  • To have a common understanding of the RISE rules, across the consortium
  • To identify researchers to be seconded and agree on the secondment plan
  • To discuss financial arrangements within the consortium and other relevant aspects of the partnership agreement (IPR, Open Access, etc.)
  • To discuss potential risks and mitigating measures
  • To identify/discuss minor changes since the submission of the proposal.

1 Day: Thursday April 27 th ,2017

11h00 : welcome
12h00 : lunch
13h30 : Kick-of meeting (1st part)

  • Introduction – Project Coordinator (5 – 10’) : Overview of IPM structuration of IPM – 4 – Citrus
  • Round table and presentation of participants :
    – Representatives names and functions
    – Lab and Industry activities
  • Common understanding and rules in RISE – summary of project officer/Project Coordinator. Tip & Tricks with participant Portal (presentation and use of participant portal during project) – Key information issued from RISE Coordination’s day (2/03/2017)
    • Mandatory actions (not flexible): mobility eligibility, element to attest the mobil ity,…
    • Secondments reflushing (project flexibility).
  • Presentations and discussions on each WP, including risks and mitigation plan – each WP leader will introduce the aims and actions in WP
    • WP1: Management and Coordination ( WP Leaders: INSAT – LISBP and CBS ) : presentation of administrative cell ; validation of funding rules,
    • WP2: Proof of concept ( WP leader: CBS )
    • WP3 : Optimisation of the new biocontrol activity ( WP leader: JKI )
    • WP4 : Economic maturation ( WP leader : BIPCA )
    • WP5: Communication, Networking and Outreach Activities and WP6: Dissemination & Exploitation (WP leader: INSAT-LISBP) : Presentation of Workspace (INSAT), Presentation of Website (INSAT).
      • Operational information about Scientific and technical actions :
      • Detailed description of Gantt [T0, T0+48months]
      • Secondments and Gantt par WP (secondments, task and sub – task)
      • Tables of IR and D, IM and MS
      • Focus on [T0, T0+18months] : Who are the partners involved? – Who are secondments ?  Who are the partner reporting?
    • Consortium agreement (CA).
      • Presentation of key – points: Administrative, financial matters, IPR, communication strategy, etc.
      • Listing/Identification of /locks/propositions/ elements to be discussed by consortium (2 nd day)

20h00: Diner in Toulouse city

2 nd Day: Friday April 28 th ,2017

9H00: Kick – off meeting (2 nd part)

  • Consortium agreement (CA)
  • Internal discussion of management, administrative and financial cells. (1 st group) (Restricted to: INSA and CBS administration, Project leaders.)
  • Toulouse’s Lab to ur (LISBP, TWB) (2 nd group)
  • Conclusions

13h00: Lunch

15h00: departure to airport (or additional lab tour)

Ce diaporama nécessite JavaScript.